Description
| Target |
Mouse IgG1 |
| Clonality |
Monoclonal |
| Clone |
R828 |
| Tested Applications |
ELISA, WB, IHC, IF/ICC, FCM, IP |
| Host |
Mouse |
| Recommended dilutions |
Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation |
Unconjugated |
| Isotype |
IgG1 Kappa |
| Form |
Liquid |
| Purity |
> 95% (SDS-PAGE) |
| Purification |
Purified by Protein A affinity chromatography. |
| Storage |
Store at 2-8°C. Do not freeze. |
| Buffer |
PBS solution with 15 mM sodium azide. |
| Specificity |
This mouse IgG1 kappa monoclonal antibody has unknown specificity and was chosen as an isotype control after screening on variety of resting, activated, live and fixed rat and human tissues. |
| Concentration |
1 mg/ml |
| Availability |
Shipped within 5-12 working days. |
| Note |
This product is for research use only. |
| Directions for use |
This reagent is intended to be used as an isotype control for flow cytometry analysis to establish the amount of non-specific antibody binding. For a particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 µg per ml of incubation mixture), change the conditions of the experiment to reduce the background. |
Research Articles on Mouse IgG1
