Description
Target | Urokinase-Type Plasminogen Activator (PLAU) |
Reactivity | Human |
Tested Applications | ELISA |
Recommended dilutions | Capture Antibody: 1/500 – 1/1000, Biotin-conjugated Detection Antibody: 1/500 – 1/1000, HRP-Conjugate: 1/500 – 1/1000. Optimal dilutions/concentrations should be determined by the end user. |
Reconstitution | Reconstitute the standard with 1 ml of Standard Diluent to obtain a stock standard solution of 40000 pg/ml. Further dilute by a factor of 10 to give the highest standard, 4000 pg/ml. Label tubes in preparation for the serial dilutions (2000, 1000, 500, 250, 125, and 62.5 ng/ml). Aliquot 0.5 ml of the Standard Diluent into each tube. Add 0.5 ml of the highest standard solution into the 1st tube and mix thoroughly. Transfer 0.5 ml from the 1st to 2nd tube, mix thoroughly, and so on. |
Storage | Aliquot and store at -20°C in the dark. Avoid repeated freeze/thaw cycles. |
UniProt Primary AC | P00749 (UniProt, ExPASy) |
Test Range | 62.5 pg/ml – 4000 pg/ml |
Detection Method | Colormetric |
Assay Type | Sandwich |
Sample Type | Serum and plasma. |
Availability | Shipped within 5-10 working days. |
Note | This product is for research use only. |
Directions for use | Bring all components to room temperature (18-25°C) and briefly spin or centrifuge the vials before use. Working solutions should be prepared and used immediately. Recommended Procedure:
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Research Articles on Urokinase-Type Plasminogen Activator (PLAU)